Gene Array

QPCR Array

About Gene qPCR Arrays

The qPCR Arrays are designed for profiling the expressions of pre-made or customized sets of coding-genes in various tissues or cells. The resulting differential expressions of profiled genes help researchers to identify those that are biologically significant and relevant to their research. An array can contain hundreds of primers for human genes or miRNA carefully chosen based on a thorough literature search of peer-reviewed publications.

How Does the Assay Work?

In a typical 96-well plate there are up to 84 pairs of qPCR primers and 12 wells of controls which are used to monitor the efficiency of the entire experimental process- from reverse transcription to qPCR reaction. Each pair of primers used in the qPCR arrays has been experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted mRNA.

From the RNA/miRNA provided, first-strand cDNA is synthesized through reverse transcription which is later mixed with the qPCR reaction mix and distributed into the wells of the array and followed by the qPCR cycling. The qPCR result is analyzed with a Data Analysis System using the ΔΔCT method to perform fold-change analysis or simple statistical analysis of the expression level (CT or CP values) for each gene.

Assay Benefits

  • Validated mRNA or miRNA primers: each primer pair is designed using a proprietary algorithm and has been experimentally validated. Primers validated for human, mouse, and rat are available- but primers for other species can be designed
  • Robust performance
  • Sensitive: detects as low as 4 copies of RNA; miRNA from as little as 10 pg of small RNA; or 20 pg of total RNA
  • Broad linearity: simultaneously detects mRNAs at different expression levels
  • Reproducible: high reproducibility for inter-array and intra-array replicates
  • Pre-arranged (cancer or pathway-related groups) or customized groups for focused study
  • Flexible assay design: arrays are available in 96-well and 384-well plates, with different layouts to choose form depending on the number of genes and replicates to be analyzed

Sample Requirements

  • Minuscule samples: few cells or small piece of tissues
  • Compatibility: works with a variety of sample types, such as cultured cells, whole blood, dried blood spots, fresh or frozen animal or plant tissues, purified RNA and heavily degraded and cross-linked RNA in formalin-fixed, paraffin-embedded (FFPE) tissues, etc.

Assay Benefits

  • Validated mRNA or miRNA primers: each primer pair is designed using a proprietary algorithm and has been experimentally validated. Primers validated for human, mouse, and rat are available- but primers for other species can be designed
  • Robust performance
  • Sensitive: detects as low as 4 copies of RNA; miRNA from as little as 10 pg of small RNA; or 20 pg of total RNA
  • Broad linearity: simultaneously detects mRNAs at different expression levels
  • Reproducible: high reproducibility for inter-array and intra-array replicates
  • Pre-arranged (cancer or pathway-related groups) or customized groups for focused study
  • Flexible assay design: arrays are available in 96-well and 384-well plates, with different layouts to choose form depending on the number of genes and replicates to be analyzed

Service Features

  • High throughput: 96 and 384 -well formats, up to 80 X 384 readouts
  • Simultaneous quantitation: assay both mRNA and protein levels in cells or cell culture supernatants
  • Highly reproducible
  • Flexible: we can quantify any gene/ protein of interest. Targets of interest and their layout are custom designed so we can deliver data from as few or as many samples / replicates as needed
  • State-of-the-art platforms: Luminex-200, FlexMAP 3D; VorTemp™ 56 Shaking Incubator or Hybridization Oven
  • Large inventory of verified genes: over 20,000 genes can be mixed to create pathway- and disease-themed panels, custom probes designed to any sequence within a few days
  • Extensive data analysis
  • Timely data delivery: 1-4 weeks or sooner- dependent upon receiving test samples
  • 30+ years of accumulated experience: Expert data analysis and interpretation, high quality scientific and technical support
  • Optional tests
    • We offer qPCR for validation of any gene of interest
    • RNA samples can be checked for integrity and quality (IQ assay) before gene array analysis
    • We have a library of >4000 validated antibodies to examine samples at protein levels using quantitative Automatic WB.
    • We offer ~100 human cancer cell lines and many types of primary cells for testing drugs and biologicals for specific projects

Why Choose Multiplex Quantigene Assay over qPCR?

Multiplex Quantigene Assay Data Examples