Description

1. Description

EmerTher® Magnetic PCR Product Purification Kit are intended for fast and simple purification of PCR products (> 100 bp).

DNA fragments from PCR reactions selectively bind to EmerTher’s unique superparamagnetic beads so that PCR by-products, such as primers, dimers, enzymes, unincorporated dNTPs and other contaminants, are removed during washing steps.

Highly purified DNA is then eluted with low salt elution buffer or water and can be used directly for downstream applications, including sequencing (Sanger and next-generation sequencing), restriction digestion, in vitro transcription, and other subsequent experiments.

2. Features

•  Completely removal of primers to avoid the interference of primers on sequencing

•  Completely removal of proteins and dNTPs

•  High recovery of PCR products (> 100 bp): 75-95%

•  High-throughput: no centrifugation or filtration, and the whole procedure can be performed by batches (96 samples per run)